Lectin Methods and Protocols

Lectins have in the past been regarded by many scientists as curious proteins of uncertain structure and specificity that bind to carbohydrates of dubious significance themselves. All this is rapidly changing. The functional importance of glycosylation in cell-cell and cell—pathogen interactions, as well as intracellular events, has been recognized by the explosion of the science of glycobiology. This has been paralleled by the realization that lectins, once they have been well characterized, can be extremely useful tools for examin­ing structural changes in glycosylation and their functional consequences for human pathophysiology.

Different lectins vary considerably in their degree of specificity. Some, such as wheatgerm agglutinin, have fairly broad specificity (for glucosamine or sialic acid), whereas others, such as Maackia amurensis, are specific not only for a single carbohydrate, but also for its linkage (2—3 linked sialic acid). Lectins with relatively broad specificity may be very useful as an adjunct to isolation or quantification of soluble glycoproteins, whereas lectins of known, and precise, specificity will be more useful for characterization of carbohy­drate structure. We have included an appendix in Lectin Methods and Proto­cols that provides the known specificities of all lectins cited in the text.

Lectin Methods and Protocols describes the use of lectins, mainly of plant origin, in three broad areas of investigation: (1) analysis of carbohy­drates, (2) isolation and quantification of glycoproteins, and (3) reaction with living cells. Techniques are described for analysis of carbohydrate structures within tissues by light or electron microscopy, quantitative and qualitative analysis of soluble glycoproteins, separation of human bone marrow cells for clinical use, and assessment of the biological effects of plant lectins.

We hope that these practical accounts of a wide range of applications of lectins will encourage further application and development of this useful and fascinating area of glycobiology.

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